Fig. 4
The expression levels of Notch signaling pathway in EGFL8 overexpressed or depleted HCC cells. a Western blot results showed the expression of Notch1, NICD, Hes1, and Hey1 in HCCLM3EGFL8 cells was obviously lower than that in HCCLM3Vecter cells. All the data were showed as mean ± SEM. ***, P < 0.001, versus HCCLM3Vector cells. Full-length blots are presented in Supplementary Fig. 2. b Western blot results showed the expression of Notch1, NICD, Hes1, and Hey1 in Hep3BshEGFL8 cells was markedly higher than that in Hep3BshCtrl cells. However, the expression levels of Notch1 signaling pathway in Hep3BshEGFL8 cells were significantly decreased after DAPT treatment. All the data were showed as mean ± SEM. *, P < 0.05, versus Hep3BshCtrl cells; ***, P < 0.001, versus Hep3BshCtrl cells; ##, P < 0.01, versus Hep3BshEGFL8; ###, P < 0.001, versus Hep3BshEGFL8. Full-length blots are presented in Supplementary Fig. 2