Fig. 3
EGFL8 overexpression inhibits the metastasis of HCCLM3 cells in vivo. a The presentative images for the mouse xenograft tumors came from HCCLM3Vecter and HCCLM3EGFL8 cells respectively. The scales in the images represent 1 cm. b No significant difference was found in the weights of xenograft tumors between HCCLM3Vecter and HCCLM3EGFL8 groups. N.S., no significance. c There was no obvious difference in the growth curves of the xenograft tumors between HCCLM3Vecter and HCCLM3EGFL8 groups. “Days” under the X axis means the time after the emergence of the subcutaneous tumors. N.S., no significance. d No difference was found in the body weights of the mice between HCCLM3Vecter and HCCLM3EGFL8 groups. “Days” under the X axis means the time after the emergence of the subcutaneous tumors. N.S., no significance. e The lung metastasis rate of HCCLM3EGFL8 cells was obviously lower than that of HCCLM3Vecter cells. Black arrow in the image indicates the metastatic HCCLM3 cell mass in lung tissues of the mice. Magnification, × 400. N.S., no significance; *, P < 0.05. f The representative results of H&E staining and immunohistochemistry assay for the expression of EGFL8 and Notch1 in xenograft mouse tumors generated from HCCLM3Vecter and HCCLM3EGFL8 cells. Magnification, × 400