Fig. 4
From: Co-relation with novel phosphorylation sites of IκBα and necroptosis in breast cancer cells
Phosphorylation of Ser-63 and 262 of IκBα plays a crucial role in cell proliferation. a S63A and S262A were constructed by introducing point mutations that replaced serine with alanine. b Transcriptional activity of p65 (i.e., activity of the NF-κB pathway) decreased in the S63A, S262A, and S63A/S262A mutants (*p < 0.05, **p < 0.001 vs. negative control). c Viability of the breast cancer cell lines MCF7 and MDA-MB 231 was reduced in the S63A and S262A mutants (**p < 0.001 vs. negative control). d Proliferation of breast cancer cell lines MCF7 and MDA-MB 231 was significantly reduced in the S63A and S262A mutants (NC vs **p < 0.001)