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Fig. 2 | BMC Cancer

Fig. 2

From: Co-relation with novel phosphorylation sites of IκBα and necroptosis in breast cancer cells

Fig. 2

Identification of phosphorylation of serine 63 and 262 on IκBα using nanoLC-quadrupole orbitrap MS/MS. a Novel phosphorylated serine 63 residue of IκBα was activated by AurkC. LC-quadrupole orbitrap MS/MS spectra of precursor ion m/z 866.4101 (charge state 2+) representing the phospho-peptide of sequence 53-LEPQEVPRGpSEPWK-68. The presence of a yn, yn-98 and bn-98 series identifies phosphorylation at serine 63. b Novel phosphorylated serine 262 of IκBα activated by AurkC. LC-MS/MS spectra of precursor ion m/z 1170.5469 (charge state 2+) representing the phosphopeptide of sequence 245-VTYQGYSPYQLTWGRPpSTR-265

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