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Fig. 5 | BMC Cancer

Fig. 5

From: The small-molecule protein ligand interface stabiliser E7820 induces differential cell line specific responses of integrin α2 expression

Fig. 5

Semi-quantitative reverse transcription PCR (sqRT-PCR) of CAPERα isoform expression in MC3T3, eEnd2, Saos-2 cells and HUVECs under standard culture conditions. a Image of a representative agarose gel of three independent repeats. b Intensity profiles of the lanes of the agarose-gel as obtained using the ImageJ gel analysis functionality. c Quantification of band intensities as strongly positive (+++, ≥ 2/3 of maximal intensity), positive (++, < 2/3 and ≥ 1/3 of maximal intensity), weakly positive (+, < 1/3 of maximal intensity) and negative (−, no band detectable). Yellow annotations were used for the n-terminal primer pairs and red annotations were used for the MII-2 domain primer pairs

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