Fig. 3

Effect of E7820 on integrin α2 expression. a Relative integrin α2 mRNA expression as determined by qRT-PCR after 17 h incubation with 50 ng/ml E7820 and 100 nM PMA or 50 ng/ml E7820 + PMA 100 nM PMA respectively, quantified using GAPDH and HPRT as housekeeping controls relative to solvent control (DMSO). b Relative integrin α2 protein expression as determined by quantitative fluorescent linked western blot analysis after 3 days of incubation with DMSO control, 50 ng/ml E7820, 100 nM PMA and 50 ng/ml E7820 + PMA 100 nM PMA and exemplary integrin α2 western blot. The full blot is shown in Figure S1. c Dose response relationship between E7820 concentration and integrin α2 mRNA expression in MC3T3 cells quantified as described above. d Change in expression of the collagen binding integrins α1, α2, α10 and α11 in response to 50 ng/ml E7802. All data is the mean of ≥3 independent repeats +1SD. * p < 0.05, ** p < 0.01, *** p < 0.001