Fig. 1

KDM6B expression was significantly increased in metastatic ESCC. a TCGA data shows the expression levels of KDM6B in 182 ESCC tissues and 286 normal esophageal tissues. b Relative levels of KDM6B in 41 surgical specimens of ESCC and matched adjacent nonmalignant tissues were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). CA, ESCC tissues; PCA, normal esophageal tissues. c,d Means of KDM6B relative levels from ESCC tissues including a group of 19 ESCC patients with positive lymph node metastases compared with another group of 22 ESCC patients with negative lymph node metastases, and representative IHC images(d). e, f Western blot and RT-qPCR data for KDM6B protein and mRNA expression in Normal esophageal epithelial cells and seven ESCC cell lines. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) serves as an internal normalized reference for KDM6B expression levels.* p < 0.05, ** p < 0.01, *** p < 0.001. ESCC, esophageal squamous cell carcinoma