Fig. 2

Wild type, but not C66Y EpCAM, inhibits tumor cell invasion in vitro and in vivo. a WHIM-3 and PyMT BO-1 breast cancer cell lines were transduced with GFP, wild type EpCAM, or C66Y EpCAM. Invasion was assessed in vitro using Matrigel chambers. b B16-F10 cells were transduced with GFP, wild type EpCAM, or C66Y (EpCAM colony 1 and 2). Protein expression is shown by immunoblot (left). Invasion (right) was assessed in vitro using Matrigel chambers. c B16-F10 cells were transduced with GFP, wild type EpCAM, or C66Y EpCAM. Transduced cells were injected into animals by tail vein, and the lungs were harvested 2 weeks later. Representative lungs samples are shown (left). Lung metastases were counted using a dissecting microscope by two independent researchers (right). All experiments were performed at least three times. Full-length blots are presented in Supplementary Fig. 5