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Fig. 2 | BMC Cancer

Fig. 2

From: Overexpression of TNFα induces senescence, autophagy and mitochondrial dysfunctions in melanoma cells

Fig. 2

Autophagy is induced in engineered melanoma cells A375hTNFa and M4BeuhTNFa. a Detection of autophagic flux by CYTO-ID autophagy detection reagent; flow cytometry, means of triplicates + SDs, unpaired t-test was used to statistical analysis, *p < 0.05. b Representative images of green fluorescent autophagic cells stained with CYTO-ID; fluorescent/light-microscope images from IncuCyte ZOOM Kinetic Imaging System, scale bar: 300 μm

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