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Fig. 1 | BMC Cancer

Fig. 1

From: Overexpression of TNFα induces senescence, autophagy and mitochondrial dysfunctions in melanoma cells

Fig. 1

Extensive mRNA overexpression of TNFα gene in engineered tumor cells does not change significantly the expression of main receptor TNFR1 and did not decrease the ability to proliferate. Cells can overcome slight caspase induction. Reverse transcriptase quantitative PCR for TNFα gene (a) and receptors TNFR1, TNFR2 (b); expression was normalized to the HPRT reference gene, values are means of triplicates or quadruplicates ± SDs; unpaired t-test was used to statistical analysis. c Continual monitoring of cell proliferation; IncuCyte ZOOM Kinetic Imaging System; means of octaplicates ± SDs; unpaired t-test was used to statistical analysis of confluence at hour 144. d Specific apoptosis induction monitoring (caspase 3/7 activation); IncuCyte ZOOM Kinetic Imaging System; means of octaplicates ± SDs; 100 ng/ml hTNFa – parental cells treated with recombinant human TNFα protein. A375, M4Beu – melanoma cells, HT29, HCT116 – colorectal carcinoma cells

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