Fig. 2

LINC_00355 silencing inhibited the proliferation and promoted apoptosis in GC cells. After HGC-27 and MKN45 cells were transfected with si-LINC_00355–1 and − 2 for 24 h, relative LINC_00355 expression levels in HGC-27 and MKN45 cells were detected by quantitative real-time PCR (a); The cell viability were measured by Cell Counting Kit-8 assay (b); Cell cycle distribution and apoptosis were measured by flow cytometer (c and d); Relative pro/cleaved caspase 3, pro/cleaved PARP, cyclin D1, and cyclin E protein levels were assessed using western blot analysis (e). All values were expressed as mean ± standard deviation. n = 3. *P < 0.05 compared with si-NC group. GC, gastric cancer; PARP, poly (ADP-ribose) polymerase; NC, negative control