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Fig. 3 | BMC Cancer

Fig. 3

From: Orthotopic model of lung cancer: isolation of bone micro-metastases after tumor escape from Osimertinib treatment

Fig. 3

Evaluation of intercostal and intratracheal administration for setting up an orthotopic model of NSCLC tumors in mice. NSCLC Luc+ cells were injected by the intercostal (a, b and c) or intratracheal (d, e and f) route in immunodeficient mice. Tumor growth was assessed by bioluminescence. a & d Results show the evolution of bioluminescence intensity over time and are expressed in cpm/cm2 for the thoracic area (mean ± SEM, n = 5). b & e Pictures show representative bioluminescence results of NSCLC Luc+ tumor-bearing mice. c & f At a late stage in tumor development, lungs from PC-9 tumor-bearing mice were collected and used for H&E staining and vimentin IHC staining. Pictures show representative results. Black arrows show tumor nests

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