Fig. 5

Overexpression of lncRNA FGD5-AS1 inhibits BMP pathway and EMT process in gastric cancer xenograft mouse model. a The macroscopic observation of subcutaneous tumor lesions and representative isolated tumor tissues from nude mice bearing xenograft tumor with normal MKN-45 cells (control group) and Overexpressed-FGD5-AS1 MKN45 cells injected. (Scale bar is shown in the image. b The average tumor weights in nude mice. (* = significant,* P < 0.05; **, P < 0.01,***P < 0.001; Student t test). c The growth curve of tumor volume. (* = significant,* P < 0.05; **, P < 0.01,***P < 0.001; Student t test). d e&g Western blot and PCR was used to show the expression of E-cadherin, N-cadherin, BMP4, t-smad1/5/8, p-smad1/5/8 and SMAD6 in tissues derived from xenograft tumors. (N = 4,* = significant,* P < 0.05; **, P < 0.01,***P < 0.001; Student t test) The original blots were shown in Fig. S7. e&f RT-PCR was used to test the expression of FGD5-AS1 and miR-196a-5p in tissues derived from xenograft tumors. (N = 4,* = significant,* P < 0.05; **, P < 0.01,***P < 0.001; Student t test)