Fig. 3

Overexpressed FGD5-AS1 sharply inhibits cancer cells proliferation, migration and epithelial-mesenchymal transition process. a Transwell assay was utilized to visually confirm metastasis inhibition function of overexpressing FGD5-AS1, and co-transfected with miR-196a-5p mimcs was used as rescue test to confirm the inhibition acting through ceRNA with miR-196a-5p. The statistics of per field cells were also illustrated the same result; Cells were calculated under microscope;The average number unber 9 fields was regarded as migaration cell amounts. (Data was expressed as mean ± SD (n = 3) * = significant, * P < 0.05; **, P < 0.01,***P < 0.005; **** < 0.001, Student t test); b) CCK-8 assay was used to test the proliferation change after FGD5-AS1 overexpressed. FGD5-AS1 group had the lowest cell viability; while co-overexpressed FGD5-AS1 and miR-196a-5p had no statistic difference compared with control group; Data was expressed as mean ± SD (n = 6) * = significant, * P < 0.05; **, P < 0.01,***P < 0.005; **** < 0.001, Student t test). c The protein level of EMT markers, E-cadherin, N-cadherin, Vimentin, MMP9 and SNAIL1, were tested by western blot in FGD5-AS1 overexpressed MKN-45 & MKN74 cell lines (The first and second lane counted from left). Co-overexpressing miR-196a-5p group used to test role of miR-196a-5p. The third lane shows no difference with control group. The original blots were shown in Fig. S4. d & e The RNA level of EMT makers, E-cadherin, N-cadherin, Vimentin, SNAIL1, were tested by western blot in FGD5-AS1 overexpressed MKN-45 & MKN74 cell lines (Data was expressed as mean ± SD (n = 6) * = significant, * P < 0.05; **, P < 0.01,***P < 0.005; **** < 0.001, Student t test)