Fig. 2
FGD5-AS1 locates in cytoplasm and binds with miR-196a-5p. a Intracellular localization of FGD5-AS1 in MKN-45 was carried out through RNA-FISH assay. The location of 18 s was used as positive control; Nuclei was stained by DAPI; 18 s rRNA (cytoplasmic positive) and FGD5-AS1 were labeled with CY3.(Scla Bar = 50 μm); b The overexpression of FGD5-AS1 was tested in MKN45 and MKN74, respectively (Data was expressed as mean ± SD (n = 3) * = significant, * P < 0.05; **, P < 0.01,***P < 0.005; **** < 0.001, Student t test); c & d The level of miR-196a-5p and the level of FGD5-AS1 in patients’ tissues has significantly negtive trend. (y = − 0.1905x + 3.448; R2 = 0.6197; Y: miR-196a-5p expression level expressed by 2-ΔΔ; X: FGD5-AS1 expression level expressed by 2-ΔΔ). e The level of miR-196a-5p was tested by QRT-PCR in overexpressed FGD5-AS1 cell lines; U6 was used as control. (Data was expressed as mean ± SD (n = 3) * = significant, * P < 0.05; **, P < 0.01,***P < 0.005; **** < 0.001, Student t test);