Fig. 3

Clonogenic Survival Assay. Quantification of clonogenic survival assay for HT29 and HCT116 cell lines after 48 h (a) or 72 h (b) treatment with 5-FU and/or Olaparib (n = 3). Representative images of clonogenic survival assay for HT29 and HCT116 cell lines after 48 h (c, d) or 72 h (e, f) treatment with 5-FU and/or Olaparib, respectively. Untreated cells were used as negative control (C). One-way ANOVA and Tukey’s post-hoc tests. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 above error bars vs. control; ^#p < 0.05 vs. 5-FU monotherapy in the same concentration