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Fig. 7 | BMC Cancer

Fig. 7

From: Dichloroacetate and PX-478 exhibit strong synergistic effects in a various number of cancer cell lines

Fig. 7

Mitochondrial respiration assay and glycolytic stress test with DCA. Figure 7 shows the results of the mitochondrial respiration assay (a) and glycolytic stress test (b) of DCA-treated MCF-7 cells performed with a Seahorse XFe96 analyzer. Three independent experiments were performed with at least four technical replicates. After measurement of the baseline OCR and ECAR, the EC50 dose of DCA was injected. After six measurement cycles, oligomycin (inhibition of ATP synthase), FCCP (uncoupling agent) and rotenone & antimycin A (inhibition of respiratory chain) were added for the mitochondrial respiration assay (a), and glucose, oligomycin and 2-DG (inhibition of glycolysis) were added for the glycolytic rate assay (b). Three measurement cycles were performed after each chemical was added. a: As shown, DCA increased the maximal OCR and thereby the maximal respiration of MCF-7 cells. b: Furthermore, DCA reduced the maximal ECAR and thereby the glycolytic capacity. See the Methods section for the exact calculation procedures and definitions of glycolytic capacity, maximal respiration, non-mitochondrial OCR and non-glycolytic acidification rate. In MCF-7 cells, DCA increased the maximal respiration by 42% and decreased the glycolytic capacity by 73% (p = 0.004 and 0.0001, respectively)

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