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Fig. 1 | BMC Cancer

Fig. 1

From: Dichloroacetate and PX-478 exhibit strong synergistic effects in a various number of cancer cell lines

Fig. 1

Synergistic interactions between DCA and PX-478 in eight cancer cell lines. Figure 1 shows the dose-response curves for DCA, PX-478 and their combination in eight different cell lines as well as the respective CIs (shown to the right of each dose-response curve). Cells were seeded in 96-well plates and treated at a confluence of approximately 50%. Forty-eight hours later, an SRB (protein mass) assay was performed. If applicable, a CI was calculated with CompuSyn for each concentration. A CI of less than 0.9 indicates synergism, a CI between 0.9 and 1.1 indicates a nearly additive effect, and a CI of greater than 1.1 indicates antagonism. Approximated EC50 values were used (EC50a) at a constant EC50a:EC50a ratio. Concentrations for which the effect of combination was significantly different from that of both single compounds and the control (p ≤ 0.05, unpaired T-Test) are marked with an asterisk (*). Synergistic interactions were confirmed for all cancer cell lines, as indicated by the CI values and predominant left shifts of the curves. Without exception, the effects of the drug combination surpassed the effects of each single compound

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