Fig. 4

DDSI under varying blocking conditions in EGFR negative 9L xenografts. a Groups of 9L rat glioma tumors were subjected to the three blocking conditions (no blocking, 5% BSA for 2 min and 10% BSA for 2 min) to validate the robustness of optimized DDSI protocol. b Tumor and adipose tissue pixel intensity histograms for the untargeted (top), targeted (middle) and DDSI (bottom) were used to generate c ROC curves. AUC values were calculated for that untargeted (red), targeted (green) and DDSI (blue) ROC curves for quantitative comparison between blocking conditions