Fig. 3

DDSI performance under varying blocking conditions in low EGFR-expressing AsPC-1 xenografts. a Groups of AsPC-1 tumors were subject to varied blocking conditions (2% BSA for 10 min, 5% BSA for 1 min, 5% BSA for 2 min, 10% BSA for 1 min, 10% BSA for 2 min and no blocking) to identify the shortest blocking and corresponding washing times that maintained a robust benign and malignant tissue differentiation. All DDSI images are optimally scaled at right (0–5 A.U.) and are representative of n = 6 tumor and adipose tissue replicates. b The DDSI tumor-to-adipose (T/A) ROC AUC values calculated after the varied blocking conditions over washing times were plotted to compare the quantified results