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Fig. 2 | BMC Cancer

Fig. 2

From: Clinically relevant dual probe difference specimen imaging (DDSI) protocol for freshly resected breast cancer specimen staining

Fig. 2

DDSI performance under varying blocking conditions in high EGFR-expressing A431 xenografts. a Groups of A431 tumors were subject to varied blocking conditions (2% BSA for 10 min, 5% BSA for 1 min, 5% BSA for 2 min, 10% BSA for 1 min, 10% BSA for 2 min and no blocking) to identify the shortest blocking and corresponding washing times that maintained a robust benign and malignant tissue differentiation. All DDSI images are optimally scaled at right (0–10 A.U.) and are representative of n = 6 tumor and adipose tissue replicates. b The DDSI tumor-to-adipose (T/A) receiver operator characteristic (ROC) curve area under the curve (AUC) values calculated after the varied blocking conditions over washing times were plotted to compare the quantified results

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