Fig. 2

The percentages of immune cells in the peripheral blood of patients before IRE treatment (pre) and 1, 3, 7, 14, and 28 days post-IRE, as analyzed with flow cytometry: a CD3⁺ T cells, b CD3⁺CD4⁺ T cells, c CD3⁺CD8⁺ T cells, d Activated T cells (CD3⁺CD69⁺), e Activated CD4⁺ T cells (CD4⁺CD28⁺), f Activated CD8⁺ T cells (CD8⁺CD28⁺), g CD4⁺ naïve cells (CD4⁺CD45RA⁺), h CD4⁺ memory cells (CD4⁺CD45RO⁺), and i Regulatory T cells (CD4⁺CD25⁺CD127^low). ^*p < 0.05, ^**p < 0.01