Table 1 Antibodies used and staining conditions
Antibody | Company | Clone | Pretreatment | Buffer incubation time (min) | Antibody incubation time (min) | Dilution |
|---|---|---|---|---|---|---|
p40 | Ventana | BC28 | Tris/Borat/ EDTA, pH 8.4 | 48 | 24 | RTU |
TTF-1 | Novocastra | SPT24 | Tris/Borat/ EDTA, pH 8.4 | 56 | 24 | 1:100 |
Synaptophysin | Cell Marque | MRQ-40 | Tris/Borat/ EDTA, pH 8.4 | 48 | 24 | RTU |
Chromogranin A | Dako | polyclonal | Tris/Borat/ EDTA, pH 8.4 | 32 | 24 | 1:400 |
CD56 | Ventana | MRQ-42 | Tris/Borat/ EDTA, pH 8.4 | 40 | 24 | RTU |