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Table 1 Antibodies used and staining conditions

From: Role of Synaptophysin, Chromogranin and CD56 in adenocarcinoma and squamous cell carcinoma of the lung lacking morphological features of neuroendocrine differentiation: a retrospective large-scale study on 1170 tissue samples

Antibody Company Clone Pretreatment Buffer incubation time (min) Antibody incubation time (min) Dilution
p40 Ventana BC28 Tris/Borat/ EDTA, pH 8.4 48 24 RTU
TTF-1 Novocastra SPT24 Tris/Borat/ EDTA, pH 8.4 56 24 1:100
Synaptophysin Cell Marque MRQ-40 Tris/Borat/ EDTA, pH 8.4 48 24 RTU
Chromogranin A Dako polyclonal Tris/Borat/ EDTA, pH 8.4 32 24 1:400
CD56 Ventana MRQ-42 Tris/Borat/ EDTA, pH 8.4 40 24 RTU
  1. CD cluster of differentiation, TTF-1 thyroid transcription factor 1