Fig. 1From: Analytical validation of the Percepta genomic sequencing classifier; an RNA next generation sequencing assay for the assessment of Lung Cancer risk of suspicious pulmonary nodulesWorkflow of Percepta GSC. Total RNA is extracted, quantified, and used as input in a library prep procedure which enriches for the coding transcriptome. After the sample is sequenced, a Percepta GSC score is calculated from an ensemble of machine learning algorithms utilizing clinical and genomic features. The Percepta GSC score is used to refine a patient’s pre-test risk stratification and a patient report is produced with the post-test risk. Assay performance was analyzed through reproducibility experiments and other studies demonstrating analytical sensitivity and analytical specificityBack to article page