Fig. 4

The antitumor efficacy of RGD4C-p21Ras-scFv in vitro. a and b Cell migration was measured with a scratch test after SW480 cells were cocultured with 20 μM RGD4C-scFv, RGD4C-linker-scFv or the anti-p21Ras scFv for 0 h, 24 h, and 48 h. The migration of SW480 cells was inhibited in the RGD4C-scFv and RGD4C-linker-scFv groups compared with the anti-p21Ras scFv and PBS control groups. c and d The migration of SW480 cells was inhibited in the RGD4C-scFv and RGD4C-scFv+CQ groups compared with RGD4C-EGFP and PBS groups. Moreover, the migration inhibition effect of RGD4C-scFv+CQ groups was higher than RGD4C-scFv group. e-h There were no difference the migration of CACO-2 and CCD841 cells in the RGD4C-scFv and RGD4C-scFv+CQ groups compared with RGD4C-EGFP and PBS groups