Fig. 4
Mechanisms by which the PIK3CA + HMCN1 mutation neutralizes the oncogenic characteristics of patients with gastric cancer carrying the LRP1B mutation. a Volcano plot of DEGs that served as the marginal effect factor in PIK3CA + HMCN1 dimutation combinations. STAD patients with the tri-mutation of LRP1B + PIK3CA + HMCN1 were chosen as the reference group to be compared with STAD patients with the mutation of LRP1B but without the di-mutation of PIK3A + HMCN1. Light blue dots denote downregulated genes; dark red dots denote upregulated genes; gray dots denote nonsignificant genes; b KEGG-based pathway enrichment. The blue color denotes a downregulation of the genes in the samples containing LRP1B mutation without PIK3CA + HMCN1 compared to the samples containing LRP1B + PIK3CA + HMCN1; the red color denotes an upregulation of the genes in the samples with LRP1B mutation without PIK3CA + HMCN1 compared to the samples with LRP1B + PIK3CA + HMCN1. C The Kaplan-Meier survival curves of gastric cancer patient cohorts presented with high expression of VTN > ~median value) and low expression of VTN (<~median value), high expression of NTSR1 (> ~ lower quartile value) and low expression of NTSR1 (<~lower quartile value), high expression of MAPK4 (<~median value) and low expression of MAPK4 (> ~ median value), high expression of CTH (<~median value) and low expression of CTH (> ~ median value), the significance of the effects on survival was calculated by using the log-rank test, p = 1.2 × 10− 4 for VTN, p = 5.1 × 10− 5 for NTSR1, p = 1.9 × 10− 6 for MAPK4 and p = 3 × 10− 5 for CTH. The x-axis denotes the survival time. The y-axis denotes the survival probability