Skip to main content
Fig. 1 | BMC Cancer

Fig. 1

From: Knock-down of LRP/LR influences signalling pathways in late-stage colorectal carcinoma cells

Fig. 1

Effect of siRNA-mediated knock-down of LRP/LR on telomerase and telomere-related proteins in late (DLD-1) colorectal cancer cells. (a) Upon transfection with Human-RPSA siRNA, relative hTERT mRNA expression levels decreased by 0.5-fold, when compared to non-transfected cells. esiRNA-RLUC served as the negative control. (b) Western blot showing a decrease in hTERT protein expression levels upon Human-RPSA siRNA transfection, in comparison to non-transfected cells. (c) Densitometric analysis of hTERT protein expression levels show a significant decrease by 0.4-fold in Human-RPSA siRNA transfected cells, when compared to non-transfected cells which were set to 100%. (d) Upon Human-RPSA siRNA transfection, telomerase activity significantly decreased by 0.8-fold. esiRNA-RLUC served as the negative control. This data represents three biological replicates with two technical repeats with newly transfected samples each time. (e) Western blot showing a decrease in pTERT protein expression levels upon Human-RPSA siRNA transfection, in comparison to non-transfected cells. (f) Densitometric analysis of pTERT protein expression levels reveal a significant decrease of 0.6-fold in Human-RPSA siRNA transfected cells, when compared to non-transfected cells which were set to 100%. (g) Western blot shows no change in TRF-1 protein expression levels upon Human-RPSA siRNA transfection, when compared to non-transfected cells. (h) This was confirmed through densitometric analysis where non-transfected cells were set to 100% and compared to Human-RPSA siRNA transfected cells. (i) Western blot showing a decrease in TFR-2 protein expression levels upon Human-RPSA siRNA transfection, in comparison to non-transfected cells. (j) Densitometric analysis of TRF-2 protein expression levels show that TRF-2 protein expression levels significantly decrease by 0.5-fold in Human-RPSA siRNA transfected cells, when compared to non-transfected cells which were set to 100%. β-actin was used as the loading control and the graphs are representative of an average of experiments performed in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001, significant: The One-way ANOVA and a Bonferroni corrected post-hoc t-test was used for statistical analysis. Full-length blots are presented in Supplementary Figs. S6-S10)

Back to article page