Fig. 6From: Extracellular vesicles-derived microRNA-222 promotes immune escape via interacting with ATF3 to regulate AKT1 transcription in colorectal cancerATF3 interacts with AKT1 in CRC cells. a, ATF3 expression in CRC cells after ATF3 downregulation determined by RT-qPCR; b, quantification of genes in CRC cells after si-ATF3 assessed by RT-qPCR; c, possible binding sites for ATF3 and AKT1 predicted by JASPAR (http://jaspar.binf.ku.dk/); d, the binding of AKT1 and ATF3 validated by ChIP experiments; e, AKT1 expression in CRC cells after co-culture or transfection determined by RT-qPCR; f, the association of AKT1 with ATF3 expression in CRC predicted by TCGA database. Two-way ANOVA was utilized to analyze data among multiple groups, followed by Tukey’s post hoc testBack to article page