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Fig. 1 | BMC Cancer

Fig. 1

From: RGD4C peptide mediates anti-p21Ras scFv entry into tumor cells and produces an inhibitory effect on the human colon cancer cell line SW480

Fig. 1

Prokaryotic expression of recombinant proteins. (a) The sequences of the anti-p21Ras scFv, RGD4C-scFv, RGD4C-linker-scFv, and RGD4C-EGFP were separately ligated into the pET28a (+) vector to construct recombinant expression plasmids. (b) The insertion sequences were detected by polymerase chain reaction (PCR) analysis in E. coli BL21 (DE3). (*): unoptimized coding sequence. (c) SDS-PAGE analysis showed that the molecular weight of the anti-p21Ras scFv was 34 kDa, that of RGD4C-scFv was 35 kDa, that of RGD4C-linker-scFv was 36 kDa, and that of RGD4C-EGFP was 34 kDa. Codon optimization did not change the molecular weights of the expression products

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