Fig. 4

Eppk1 lies downstream of KLF5 and promotes Hela cell proliferation. a HeLa cells were transfected with si-Con or Eppk1-specific siRNA (si-Eppk1) for 24 h. Eppk1 protein expression was analysed by western blot. β-actin was used as the loading control. Right: band intensities were measured and normalized to β-actin (n = 3). Data represent the mean ± SD. **P < 0.01 vs. si-Con. b and c Knockdown of Eppk1 significantly affected HeLa (b) and SiHa (c) cell growth compared to transfection with si-Con at different indicated time points by a CCK-8 test (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the si-Con. d Ad-GFP and Ad-KLF5-infected HeLa cells and Ad-KLF5-infected HeLa cells were transfected with si-Eppk1 for 36 h. Expression of Eppk1 was confirmed by western blot. Right: band intensities were measured and normalized to β-actin. Data represent the mean ± SD. **P < 0.01 vs. Ad-GFP. e and f CCK-8 assay for cell proliferation in Hela and SiHa cells treated as in (b) and (c) respectively. Data represent the mean ± SD. *P < 0.05 and **P < 0.01 vs. Ad-GFP