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Fig. 3 | BMC Cancer

Fig. 3

From: KLF5-mediated Eppk1 expression promotes cell proliferation in cervical cancer via the p38 signaling pathway

Fig. 3

KLF5 regulates Eppk1 expression by direct binding to Eppk1 promoter. a A schematic map of the − 1000 to + 1 bp regions of the Eppk1 promoter showing a KLF5-binding site at − 295 to − 285 bp region. b A luciferase reporter controlled by the Eppk1 promoter or the Eppk1 promoter with a mutation in KLF5-binding site was transfected into Hela cells with increasing amounts of KLF5 expression plasmid (pcDNA3.1-KLF5). PGL3-Basic was used as the negative control. Luciferase activity was monitored using the dual luciferase reporter test system. Data represent relative Eppk1 promoter activity normalized to pRL-TK activity. **P < 0.05 vs. pcDNA3.1. c Hela cells were infected with or without Ad-KLF5 for 24 h. ChIP test was then performed with an antibody against Klf5. Nonimmune IgG was served as negative control. Immunoprecipitated DNA was amplified by PCR using the primers showed as in (a)

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