Fig. 5

Mutation analysis by dPCR in (EV) RNA with and without DNAse treatment. Dot-plots in panel a-d indicate the presence of wild-type (WT) and/or mutant (MT) PIK3CA p.H1047R copies in BT20 mRNA treated a) with DNase and reverse transcriptase (RT), b) with DNAse and without RT, c) without DNase and with RT, d) without both DNAse and RT. Dot-plot in panel e-f indicate the presence of wild-type (WT) and/or mutant (MT) of EGFR p.T790M in patient 15 EV-RNA e) treated without DNAse and f) with DNAse treatment. Similar mutant and wild-type copies were observed in both conditions. Blue: wells with mutant copies, Red: wells with wild-type copies, Green: wells containing both wild-type and mutant copies, Yellow: empty wells, Grey: undetermined wells