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Fig. 4 | BMC Cancer

Fig. 4

From: Acquisition of cancer stem cell capacities after spontaneous cell fusion

Fig. 4

a. At the top, expression of NANOG and β-actin evaluated by western blotting in wild type cell lines and after 72 h of incubation with siRNA negative control or siRNA NANOG. At the bottom, quantification of NANOG expression normalized by β-actin expression (loading control). Full-length blots/gels are presented in Supplementary Fig. 6. b. Images of scratch test migration assay at 0 h and 10 h after inhibition of NANOG by siRNA compared to negative control siRNA. c. Evaluation of migration with scratch test for RST and H1 in wild type cell lines and after incubation with non-targeted siRNA or siRNA NANOG. *** p value < 0.001 (Mann-Whitney test) (n = 16)

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