Fig. 4
Blocking DGAT1 pathway impaired reactive oxygen species expression in gastric cancer cell lines. a Gastric cancer tissues expressed higher level of DGAT1 (n = 5, p = 0.0004). 5 × 105 GES-1, AGS or MKN45 cells were cultured in 6 well plates and/or treated with sodium oleate (200 μM) and/or A922500 (100 μM) for 12 h. Then cells were collected and mRNAs were isolated for further studies. qPCR analysis of DGAT1 (b) in gastric cancer cell lines, or in MKN45 after sodium oleate treatment (c), or NOX2 (d) and IDO (e) in MKN45 after sodium oleate and/or iDGAT treatment. *P < 0.05; **P < 0.01; ***P < 0.001. Data are representative of three independent experiments with similar results. Quantification of signal was shown in bar graphs and error bars represent mean ± SD