Table 1 Concentration ranges of the ten chemotherapeutic drugs and lovastatin in the S. cerevisiae assays
Drug | Principal mechanism of action | Concentration Range (μM) |
|---|---|---|
Cisplatin | DNA crosslinking | 12.4–105 |
Cyclophosphamide | Alkylating agent which binds to DNA | 49.4–420 |
Doxorubicin | Intercalates DNA, blocking replication | 24.7–210 |
Epothilone | Binds to microtubules, blocking cell division | 1.1–9.3 |
Etoposide | Topoisomerase II inhibitor | 0.165–1.4 |
5-Fluorouracil | Antimetabolite: blocks thymidylate synthetase | 0.92–8.07 |
Gemcitabine | Antimetabolite: incorporated into DNA in place of dCTP | 14.4–123 |
Methotrexate | Antimetabolite: binds to dihydrofolate reductase | 8.24–70.0 |
Rapamycin | mTOR inhibitor | 0.33–2.80 |
Tamoxifen | Selective estrogen receptor modulator (SERM) | 24.7–210 |
Lovastatin | Competitive inhibitor of HMG-CoA reductase | 12.7–108 |