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Table 1 MSI status using NGS-MSI against conventional PCR and IHC

From: A next-generation sequencing-based strategy combining microsatellite instability and tumor mutation burden for comprehensive molecular diagnosis of advanced colorectal cancer

PCR-MSI as Gold Standard

IHC-MMR as Gold Standard

 

PCR Positive

PCR Negative

 

IHC Positive

IHC Negative

NGS Positive

32

0

NGS Positive

32

0

NGS Negative

1

65

NGS Negative

6

60

Sensitivity = 97.0%(32/33)

Sensitivity = 84.2%(32/38)

Specificity = 100%(65/65)

Specificity = 100%(60/60)

Concordance Rate = 99.0%(97/98)

Concordance Rate = 93.9%(92/98)