Skip to main content

Table 1 MSI status using NGS-MSI against conventional PCR and IHC

From: A next-generation sequencing-based strategy combining microsatellite instability and tumor mutation burden for comprehensive molecular diagnosis of advanced colorectal cancer

PCR-MSI as Gold Standard IHC-MMR as Gold Standard
  PCR Positive PCR Negative   IHC Positive IHC Negative
NGS Positive 32 0 NGS Positive 32 0
NGS Negative 1 65 NGS Negative 6 60
Sensitivity = 97.0%(32/33) Sensitivity = 84.2%(32/38)
Specificity = 100%(65/65) Specificity = 100%(60/60)
Concordance Rate = 99.0%(97/98) Concordance Rate = 93.9%(92/98)