Fig. 3
From: Distribution of chimeric antigen receptor-modified T cells against CD19 in B-cell malignancies
CAR-T distribution in the tumor-bearing mice. a. Flow diagram of the experiment. Blood and organs were taken from the tumor-bearing mice at different times (blood: 5 mins, 30 mins, 1 h and day1; blood and organs: 3 h, day2, day7, day14, day28, day42 and day56) after CAR-T cell infusion. b. Changes of CAR-T cell distribution in the blood over time. DNA from blood was extracted and the CAR gene copies were detected by qPCR. c. Changes of CAR-T cell distribution in the brain, uterus, testis, ovary and epididymis over time. DNA from 5 organs were extracted and the CAR gene copies were detected by qPCR. d. Changes of CAR-T cell distribution in the heart, liver, spleen, lung and kidney over time. DNA from 5 organs were extracted and the CAR gene copies were detected by qPCR. e. AUC of CAR-T cells in tissues and whole blood