Fig. 4

MiR-105-3p modulates GOLIM4 expression in MCF-7 and ZR-75-30 cells by directly targeting its 3’UTR. a The predicted miR-105-3p target sequence in the 3’UTR of GOLIM4 and a Mut sequence containing altered nucleotides in the 3’UTR of GOLIM4. b Luciferase reporter assays for luciferase activities in the MCF-7 and ZR-75-30 cells synchronously transfected with miR-105-3p mimic or NC mimic together with psiCHECK2- GOLIM4–3’UTR WT or psiCHECK2- GOLIM4–3’UTR MUT. c, d RT-qPCR and western blot analysis of GOLIM4 in MCF-7 and ZR-75-30 cells after transfection with the miR-105-3p inhibitor. Asterisks indicate significant differences from the control. e The expression of GOLIM4 in tumour and control tissues as well as breast cancer cell lines. f Correlation analysis of the expression of GOLIM4 and miR-105-3p in breast cancer tissues. (**P < 0.01, Student’s t-test, compared to the NC mimic group or NC inhibitor group)