Fig. 2

High-dose doxorubicin specifically activated autophagy flux in DR-BC cells, instead of their parental DS-BC cells. a-f By conducting Western Blot analysis, we found that doxorubicin specifically increased LC3B-II/I ratio, and decreased p62 expression levels in DR-BC cells (full-length blots/gels are presented in Supplementary Fig. S5A-B). g, h Electronic microscope (EM) was used to observe the autophagosomes (indicated by the red arrows), and the magnificence of the images were 5000 ×. i-l The relative levels of LC3B-II/I ratio was determined by Western Blot analysis in DR-BC cells (full-length blots/gels are presented in Supplementary Fig. S5C-D). Each experiment repeated at least 3 times, and *P < 0.05 was regarded as statistical significance