Fig. 3From: Activation of WNT7b autocrine eases metastasis of colorectal cancer via epithelial to mesenchymal transition and predicts poor prognosisWNT7b was overexpressed in colorectal cells. a Representative images of immunofluorescence staining of WNT7b subcellular localization of FHC and HCT116 cells. Nuclei were counterstained with DAPI. Scale bar equals to 20 μm. b and c Cytoplasmic, nuclear, and membrane lysate of FHC and HCT116 cells were separately extracted and analyzed by Western Blot, with GAPDH, Lamin B1, and Na/K ATPase alpha 1 as the loading control, respectively. Quantification of the WNT7b expression was analyzed by image J, P value was shown and calculated by student’s t testBack to article page