Fig. 2
Iron decreases the transcription of clbA promoter activity promoted by oligosaccharides. a Growth curves and luminescence measurement of EcN the translational clbA luciferase reporter fusion and b area under the curve (AUC) of RLU/OD600, in media containing 5 μM, 25 μM and 125 μM ferrous sulfate without inulin and with c 20, d 30, e 40 mg/mL inulin and f-h corresponding area under the curve (AUC) of RLU/OD600. Growth curves and luminescence measurement of EcN in media containing 5 μM, 25 μM and 125 μM ferrous sulfate supplemented with i 20, j 30, k 40 mg/mL GOS and l-n corresponding area under the curve (AUC) of RLU/OD600. *P < 0.05, **P < 0.01, ***P < 0.001 compared to control (0 mg/mL oligosaccharides); &P < 0.05, &&P < 0.01, and &&&P < 0.001; ANOVA