Fig. 1

Characteristics of isolated fibroblasts from normal endometrium and endometrial cancer specimens. (A) Fibroblasts isolated from normal endometrium sample #1 (top, NF1) and those from endometrial cancer sample #1 (bottom, CAF1) were used for immunocytochemistry. Anti-pan-cytokeratin (pan-CK) and anti-VIM antibodies were used to visualize protein expression. (B) Isolated NF (n = 6) and CAF lines (n = 7) were used for immunoblotting to detect the expression of ACTA2, FAP, VIM, CDH2 and SPARC. GAPDH was used as an internal control. Full-length blot images are presented in Supplementary Fig. 1. (C) Semi-quantification of the immunoblotting data in (B). Intensity of the bands was quantified using Image J (https://imagej.nih.gov/ij/). Values of the protein-of-interest were corrected using the intensity of GAPDH bands. (D) ELISA analysis of SPARC secreted from the NF and CAF lines. (E) In vitro contraction analysis of the NF and CAF lines. The images show representative results of the experiments. The graphs on the right show quantification data of the results. The scale bars indicate 100 μm. 488, Alexa Fluor 488; pan-CK, pan-cytokeratin; NF, normal fibroblasts; CAF, cancer-associated fibroblasts; n.s., not significant; *, P < 0.05