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Fig. 6 | BMC Cancer

Fig. 6

From: ERK3 is transcriptionally upregulated by ∆Np63α and mediates the role of ∆Np63α in suppressing cell migration in non-melanoma skin cancers

Fig. 6

ERK3 knockdown does not affect cell proliferation. a HaCaT and A431 cells were stably transduced with lentiviruses expressing shRNA specifically targeting ERK3 (shERK3) or a non-targeting control shRNA (shGIPZ) as indicated. The change in protein levels of stable cells was analyzed by immunoblot analysis after 14 days of selection by puromycin. Representative western blots are shown as cropped gel images. Full length blots are presented in Additional File 5 (Figure S5). MTS cell proliferation assay was performed by measuring the number of viable cells daily for 5 days. The relative cell growth (indicated by optical density (OD) at 490 nm) of b HaCaT cells or c A431 cells expressing shERK3 or shGIPZ at different days are presented in the y-axis. Values represent mean ± S.D. The statistical analysis shows no significant difference between the shERK3 and the shGIPZ control in both cell lines as analyzed by Student’s t-test

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