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Fig. 5 | BMC Cancer

Fig. 5

From: ERK3 is transcriptionally upregulated by ∆Np63α and mediates the role of ∆Np63α in suppressing cell migration in non-melanoma skin cancers

Fig. 5

ERK3 restoration counteracts the increase in cell migration induced by ΔNp63α silencing. a A431 cells were transiently transfected for two rounds with non-silencing control siRNA (NSC) or ΔNp63α siRNA (sip63) as indicated. Along with the second sip63 transfection, cells were also transduced with lentiviral empty vector pCDH CMV-MCS-EF1-Puro (CDH) or pCDH-Myc6-ERK3 (CDH-ERK3) as indicated. Twenty-four hours after lentivirus transduction, one set of cells were harvested, and ERK3 and ΔNp63α proteins level were analyzed by immunoblot analysis. Exogenously expressed ERK3 protein with 6 myc-tags (Myc6-ERK3, the upper bands) and endogenous ERK3 protein (lower band) were indicated by arrows. Representative western blots are shown as cropped gel images. Full length blots are presented in Additional File 4 (Figure S4). The other set of cells were subjected to trans-well cell migration assay and the number of migrated cells was quantitated after 18 h. Representative images of migrated A431 cells with crystal violet staining under each condition were shown in (b). The average number of migrated cells per well is presented in the y-axis of the bar graph in (c). Values represent mean ± S.D. An asterisk indicates a statistically significant difference with P < 0.0001 by Student’s t-test

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