Fig. 3

ΔNp63α positively regulates ERK3 gene expression. a A431 and b HaCaT cells were transiently transfected with non-silencing control siRNA (NSC) or siRNA specific to p63 (sip63). Total RNA was extracted and ΔNp63α and ERK3 transcript levels were measured by qRT-PCR. Values represent mean ± S.D. of three experiments. An asterisk indicates a significant difference with P < 0.05 by Student’s t-test. c Immunoblots of ΔNp63α and ERK3 in A431 and HaCaT cells transfected with NSC or sip63. Immunoblot with β-actin was performed to confirm equivalent protein loading. Representative western blots are shown as cropped gel images. Full length blots are presented in Additional File 2 (Figure S2). d H1299 cells were co-transfected with pGL3 empty vector, p63-BS1- or p63-BS2-Luc reporter constructs along with the empty vector (EV) or 0.1 μg or 0.3 μg of ΔNp63α expressing plasmid. Cells were subjected to dual luciferase assay at 24 h post-transfection. The Y-axis represents the fold change in the luciferase activity compared to cells co-transfected with pGL3 empty vector and empty vector control (EV)