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Fig. 1 | BMC Cancer

Fig. 1

From: The role of S100A4 for bone metastasis in prostate cancer cells

Fig. 1

S100A4 expression and secretion is elevated in bone-metastatic prostate cancer cells. a Flow cytometry with anti-human β2-microglobulin Ab verified these bone-metastatic PC3 cells (mtPC3s) as human cells. Neg. Cont., mouse bone marrow cells; Pos. Cont., HEK-293 cells. b S100A4 mRNA expression in LNCaP, PC3, and mtPC3 cells was analyzed with real-time PCR. n = 3 per group. c S100A4 protein expression in LNCaP, PC3, and mtPC3 cells was analyzed by western-blotting. d Levels of S100A4 protein secretion in LNCaP, PC3, and mtPC3 cells in cell culture medium were measured by ELISA. n = 3 per group. ***p < 0.001 by one-way ANOVA with post hoc Tukey’s test. Data are presented as the mean ± SD. The full-length gels are presented in Supplementary Fig. 3a

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