Fig. 6

Off target different systemic effects. We conducted clinical, histopathologic and hemopoietic assessment 4 weeks post-treatment. a A hamster treated with CPT showing partial alopecia. b Normal liver section extracted from 5Fu-AuNSs conjugated group showing normal hepatocytes and liver parenchyma. The same images were obtained from the other 2 nanoconstructs. c Liver section showing focal necrosis near the portal vein obtained for both free cytotoxic drugs CPT and 5Fu. d, e Areas of glomerular shrinkage in hamsters receiving CPT and 5Fu. f Platelet count for the 8 groups across all time points; no significant difference was noted between the 3 nanoconjugates groups (ns) with only one exception seen for CPT-AuNSs at 48 h. A significant decrease in the platelets count was seen in both free 5Fu and CPT denoted with asterisks (p< 0.001). g RBCs count showing no significant difference between the 3 nanoconjugates groups (ns) (p< 0.001). h WBCs values also show no significant difference between the 3 nanoconjugates groups (ns) (p< 0.001), while a significant fluctuation was seen for WBCs count in both cytotoxic drugs denoted with asterisks (p< 0.001). i For haemoglobin, no significant difference was found across all groups except the unconjugated form of FGFR1i denoted with asterisks (p< 0.001). (n=13)