Fig. 4

NaB inhibits the migration and invasion capacity of CRC cells. The wound healing assay and transwell invasion assay were used to evaluate the migration and invasion capacity, respectively, of the HCT8 and HCT116 cells. The wound distances of HCT8 cells untreated with NaB at 0 h (A1), HCT116 cells untreated with NaB at 0 h (A2), HCT8 cells untreated with NaB at 24 h (B1), HCT116 cells untreated with NaB at 24 h (B2), HCT8 cells treated with NaB at 0 h (C1), HCT116 cells treated with NaB at 0 h (C2), HCT8 cells treated with NaB at 24 h (D1), and HCT116 cells treated with NaB at 24 h (D2) were photographed. The statistical analysis of the wound distance of HCT8 cells and HCT116 cells is shown in panels E1 and E2, respectively (Student’s t test). The invading HCT8 cells untreated with NaB (A3), HCT8 cells treated with 5 mmol/L NaB (B3), HCT116 cells untreated with NaB (A4), and HCT116 cells treated with 5 mmol/L NaB (B4) were photographed under a microscope; the statistical analysis is shown in panel E3