Fig. 2

NaB induces apoptosis of CRC cells. NaB was determined by flow cytometric analysis of FITC-Annexin V and propidium iodide (PI) staining. The number of cells in each quadrant in this figure are representative of (Q1) necrosis, (Q2) late apoptosis, (Q3) live cells, and (Q4) early apoptosis. The panels A1–4, panels B1–4, panels C1–4, and panels D1–4 represent the SW480, LOVO, HCT116, and HCT8 cell lines. The panels A1, B1, C1, and D1 represent the cells untreated with NaB (control group). The panels A2, B2, C2, and D2 represent the cells treated with 2.5 mmol/L NaB. The panels A3, B3, C3, and D3 represent the cells treated with 5 mmol/L NaB. The panels A4, B4, C4, and D4 represent the cells treated with 10 mmol/L NaB