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Fig. 4 | BMC Cancer

Fig. 4

From: Distinct roles for the RNA-binding protein Staufen1 in prostate cancer

Fig. 4

Staufen1 knockdown inhibits Prostate Cancer cell invasion and motility. Motility and Invasion assays were performed following 48 h of Control (CTL) or Staufen1-shRNA (shStau1) expression. a Western blot analysis of Staufen1 expression showing a representative blot of Staufen1 with β-actin as a loading control in PC3 and DU145 cells. Each representative blot is cropped to show an n = 1 for each cell line from their respective full-length blot. Note that the DU145 cells used for Fig. 4 were also used for Fig. 5 experiments and therefore a different representative image from the same blot was selected for each figure. Quantification of n = 4 is represented normalized to CTL. Data are Mean ± SD, One-Sample T-Test, **P < 0.01, ***P < 0.001. b Cells were seeded into transwell chambers containing membranes coated with or without Matrigel and incubated for 72 h and 24 h for PC3 and DU145 cells, respectively. Representative images of cells that passed through the transwell chamber at 40X magnification, scale bar = 20 μm, are displayed. Average number of cells/field of view is quantified for cell motility (no matrigel) and invasion (matrigel) as a percentage relative to CTL. Quantifications are for all data are n = 4. Data are Mean ± SD, Students T-Test, **P < 0.01, ***P < 0.001. Full length blots are presented in Supplemental Fig. 6

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